Feb 07, 2019 To accomplish this, the serial dilution–agar plate technique is used. Briefly, this method involves serial dilution of a bacterial suspension in sterile water blanks, which serve as a diluent of known volume. Once diluted, the suspensions are placed on suitable nutrient media. The pour-plate technique is the procedure usually employed. What are the advantages of the serial dilution agar plate procedure. Disadvantage: the method requires an incubation periods so it takes longer to get results. Serial dilution method for bacterial. Diluted bacteria samples then spread onto the agar plate by L-shaped. The advantages and disadvantages of serial. You can determine the number of.
- Advantages Of Serial Dilution-agar Plate Procedure
- Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Pdf
Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution.
Principle of Spread Plate Technique
- The disadvantage of this isolation method is 'within the agar.' (a) Spread plate (b) Smear plate (c) Streak plate (d) Pour plate (e) serial dilution 100. The disadvantage of this isolation method is 'flaming students.'
- What is the major just disadvantage of microbial counts for performed by methods other than serial dilution-agar plate procedure? Advantage - it has countable viable cells to count Disadvantage - spread and pour plates might not always have a single colony that represents the progeny of a single cell.
The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
Procedure of Spread Plate Technique
- Make a dilution series from a sample.
- Pipette out 0.1 ml from the appropriate desired dilution series onto the center of the surface of an agar plate.
- Dip the L-shaped glass spreader into alcohol.
- Flame the glass spreader (hockey stick) over a Bunsen burner.
- Spread the sample evenly over the surface of agar using the sterile glass spreader, carefully rotating the Petridish underneath at the same time.
- Incubate the plate at 37째C for 24 hours.
- Calculate the CFU value of the sample. Once you count the colonies, multiply by the appropriate dilution factor to determine the number of CFU/mL in the original sample.
Advantages Of Serial Dilution-agar Plate Procedure
Counting bacterial colonies on agar plates is a simple and effective method for determining the number of viable bacteria in a sample. This method relies on the growth of a bacterial cell in an agar plate to form a visible colony, only living or viable bacterial cells will be counted.
Uses of Spread Plate Technique
- It is used for viable plate counts, in which the total number of colony forming units on a single plate is enumerated.
- It is used to calculate the concentration of cells in the tube from which the sample was plated.
- Spread plating is routinely used in enrichment, selection, and screening experiments.
Limitations of Spread Plate Technique
![What are some advantages and disadvantages of the serial dilution agar plate technique What are some advantages and disadvantages of the serial dilution agar plate technique](https://www.coursehero.com/doc-asset/bg/bb450e1a96f9c1d7fcb3ff9afa92f5bb189147ca/splits/v9.1.frq/split-0-page-1-html-bg.jpg)
- Strick aerobes are favored while microaerophilic tends to glow slower.
- Crowding of the colonies makes the enumeration difficult.
References
![Disadvantage Disadvantage](https://i2.wp.com/microbeonline.com/wp-content/uploads/2016/10/Pouring-the-molten-agar-medium-300x156.png?resize=385%2C200)
Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Pdf
- M. J. Pelczar
- Mackie and McCartney. Practical Medical Microbiology
- Bailey & Scott’s Diagnostic Microbiology.
- Practical Microbiology. Pradeep Kumar Sharma.